Kmmig1 as a disrupted mutant of mig1 encoding a regulator for glucose repression in kluyveromyces marxianus exhibits a histidineauxotrophic. Genolevures is a largescale comparative genomics project between saccharomyces cerevisiae and other yeast species representative of the various branches of the hemiascomycetous class and provides annotated sequence data and classifications for the genomes of eighteen. Kluyveromyces marxianus, thermotolerant yeast, complete genome sequence, transcriptome analysis, xylose fermentation background along with rising concern about global warming and the rapid increase in fuel consumption, there is worldwide interest in. Kluyveromyces marxianus is a sister species to the betterknown k. It is the sexual stage of atelosaccharomyces pseudotropicalis also known as candida pseudotropicalis. Academic research paper on topic genetic basis of the highly efficient yeast kluyveromyces marxianus. Apr 15, 20 kluyveromyces marxianus combines the ease of genetic manipulation and fermentation with the ability to efficiently secrete high molecular weight proteins, performing eukaryotic posttranslational modifications. Comparison of the deduced aminoacid sequence of this gene to that of the saccharomyces cerevisiae pdc genes reveals extensive homology including a motif common to thiamin diphosphate. Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. Kluyveromyces marxianus combines the ease of genetic manipulation and fermentation with the ability to efficiently secrete high molecular weight proteins, performing eukaryotic posttranslational modifications. A yeast first described by ec hansen in 1888 and named saccharomyces marxianus after marx, the person who originally isolated k. Kluyveromyces marxianus is a thermotolerant and efficient xyloseutilizing yeast. However, efficient coutilization of glucose and xylose from the lignocellulosic biomass is a challenge due to the glucose repression in microorganisms. Wo19940821a1 the use of the kluyveromyces marxianus.
When transformed with both the pkcas plasmid and a donor dna, homologous. Fermentation of dough with an inulinasesecreting kluyveromyces marxianus strain allowed to reduce fructan levels in the final product by more than 90%, while only 56% reduction was achieved when a control saccharomyces cerevisiae strain was used. Lignocellulosic biomass is one of the most abundant materials for biochemicals production. Additionally, the presence of dna binding sites for the same transcription factor may coordinate expression of multiple genes. Draft genome sequence of the probiotic yeast kluyveromyces. Genome sequences are available for all six kluyveromyces species, including k. It is able to grow efficiently in a wide range of temperatures. Biological parts for kluyveromyces marxianus synthetic. Kluyveromyces marxianus, thermotolerant yeast, complete genome sequence, transcriptome analysis, xylose fermentation background along with rising concern about global warming and the rapid increase in fuel consumption, there is worldwide interest in the production of bioethanol from renewable resources 1. The isolation and nucleotide sequence of the pyruvate. In the present study, the ygl157w gene product kmgre2 was purified to. The genome described here is composed of 11,165,408 bases and has.
Kluyveromyces marxianus strain dmku31042 bcc 29191. The invention provides a nucleic acid sequence derivable from a yeast, e. Complete genome sequence of kluyveromyces marxianus nbrc1777. Kluyveromyces marxianus, the known fastestgrowing eukaryote on the earth, has remarkable thermotolerance and capacity to utilize various agricultural residues to produce lowcost bioethanol, and hence is industrially important to resolve the imminent energy shortage crisis. Characterization of the kluyveromyces marxianus strain dmb1. In saccharomyces cerevisiae, the alcohol dehydrogenase genes adh1 and adh5 are part of a duplicated block of genome, thought to originate from a genomewide duplication posterior to the divergence from the kluyveromyces lineage. Draft genome sequence of kluyveromyces marxianus strain dmb1. We have determined the nucleotide sequence of a 2360basepair bp region of the kluyveromyces marxianus genome containing the structural gene for the enzyme pyruvate decarboxylase pdc. Transcriptional analysis of kluyveromyces marxianus for. One major limitation is the lack of comprehensive genome sequence information and there is still a strong reliance on a partial genome sequence of strain cbs 712 llorente et al. Gene regulation in kluyveromyces marxianus in the context of. Here, we engineered the thermotolerant yeast kluyveromyces marxianus to create a new synthetic biology platform. The genome of strain nbrc1777 is composed of 4,912 open reading frames orfs on 8 chromosomes, with a total size of 10,895,581 bp, including mitochondrial dna. This species has a homothallic mating system and is often isolated from dairy products.
Kluyveromyces marxianus developing ethanol tolerance. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. Kluyveromyces marxianus is a thermotolerant yeast that has been explored for potential use in biotechnological applications, such as production of biofuels, singlecell proteins, enzymes, and other heterologous proteins. Development of a comprehensive set of tools for genome. Like in its close relative, kluyveromyces lactis, this requires lactose transport via a permease and intracellular hydrolysis of the disaccharide. Jul 19, 2014 eureka, we are back to spoilage yeasts. This study revealed the presence of interesting features in k. Intraspecies polymorphisms of kluyveromyces marxianus. Three conditions were considered from the downloaded dataset. The genome described here is composed of 11,165,408 bases and has 4,943 proteincoding genes.
Origin of lactose fermentation in kluyveromyces lactis by. One of the important traits that sets it apart from the familiar yeasts, saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Kluyveromyces lactis formerly saccharomyces lactis is a yeast which has the ability to assimilate lactose and convert it into lactic acid. A comparison of its genomic information with those of other yeasts and transcriptome analysis revealed that the yeast bears beneficial properties of temperature resistance, widerange bioconversion ability, and production of recombinant. Draft genome sequence of kluyveromyces marxianus strain. Complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast article pdf available in genome announcements 32. Thermal adaptability of kluyveromyces marxianus in. Genetic and physiological basis for antibody production by. Comparison of the deduced aminoacid sequence of this gene to that of the saccharomyces cerevisiae pdc genes reveals extensive homology including a motif common to thiamin diphosphatedependent enzymes. Frontiers ploidy variation in kluyveromyces marxianus. This article is from genome announcements, volume 2. Kluyveromyces marxianus in ascomycetous yeast and member of the genus, kluyveromyces. Using crisprcas9 clustered regularly interspaced short palindromic repeats with cas9mediated genome editing, we show that wild isolates of k. Abstractwe determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1.
Kluyveromyces marxianus developing ethanol tolerance during. The genome is composed of 8 chromosomes, with a total size of 11. The open reading frame ygl157w in the genome of the yeast kluyveromyces marxianus strain dmb1 encodes a putative uncharacterized oxidoreductase. Complete genome sequence of kluyveromyces marxianus. A comparison of its genomic information with those of other yeasts and transcriptome analysis revealed that the yeast bears beneficial properties of temperature resistance, widerange bioconversion ability. The sequences in question were identified from the genome sequence data assembly gca 000299195. The order of these six genes reflects the structure of the ancestral s. Its name comes from the ability to assimilate lactose and convert it into lactic acid. Characterization of the kluyveromyces marxianus strain. In contrast, scientific literature about the fundamental aspects of k. While the wildtype yeast is already in use for producing fragrances and fermented products, the lack of standardised tools for its genetic and metabolic engineering prevent it from being used as a nextgeneration cell factory for biobased chemicals. Genetic basis of the highly efficient yeast kluyveromyces marxianus. Saccharomyces cerevisiae and kluyveromyces marxianus. Complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast article pdf available in genome announcements 32 april 2015 with 145 reads.
We determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. Alcohol dehydrogenases from kluyveromyces marxianus. Polymorphisms in the lac12 gene explain lactose utilisation. Currently, the poor ethanol tolerance hinders its operable application in the industry, and it is necessary to. To realize the glucosexylose coutilization, analyzing the glucose. Kluyveromyces lactis is a kluyveromyces yeast commonly used for genetic studies and industrial applications. Reconstruction and analysis of a kluyveromyces marxianus genome. Kluyveromyces marxianus strain dmku31042 bcc 29191 nbrc 104275 mutations.
Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse nondairy environments. Mig1 as a positive regulator for the histidine biosynthesis. Regulatory regions comprise a promoter, a terminator, and a sequence. Pdf complete genome sequence of kluyveromyces marxianus. To ensure sufficient co2 production, cocultures of s. Genome sequence of the thermotolerant yeast kluyveromyces. Today, i would like to introduce another spoilage yeast called kluyveromyces marxianus anamorph candida kefyr.
Kluyveromyces marxianus exhibits an ancestral saccharomyces. However, this protein shows 46% identity with the saccharomyces cerevisiae s288c nadphdependent methylglyoxal reductase, which exhibits broad substrate specificity for aldehydes. Comparison to cbs2359 reveals genomewide nucleotide sequence identity of 95. This copy of the genome of kluyveromyces lactis strain clib210 was obtained from genolevures release version. Frontiers biological parts for kluyveromyces marxianus. Mar 22, 2019 kluyveromyces marxianus, the known fastestgrowing eukaryote on the earth, has remarkable thermotolerance and capacity to utilize various agricultural residues to produce lowcost bioethanol, and hence is industrially important to resolve the imminent energy shortage crisis. Crisprcas9 genome editing and matingtype switching in k. We report here the characterization of kluyveromyces marxianus adh2 and the five genes found in its immediate downstream region, mrps9, yol087c, rpb5, rib7 and spp381. Here, we report the draft genome sequence of kluyveromyces marxianus fragilis b0399, the first yeast approved as a probiotic for human consumption not belonging to the genus saccharomyces. Release of glucose repression on xylose utilization in.
Jeong h, lee dh, kim sh, kim hj, lee k, song jy, kim bk, sung bh, park jc, sohn jh, koo hm, kim jf 2012 genome sequence of the thermotolerant yeast kluyveromyces marxianus var. The secretion performances were analyzed in the host k. Dna sequence 1 refseqnuc 1 protein domain 1 pfam 1 all databases 17 download rdf. Pdf draft genome sequence of kluyveromyces marxianus strain. Kluyveromyces marxianus strain dmku31042 bcc 29191 nbrc 104275 expression system. Genome sequence of the thermotolerant yeast kluyveromyces marxianus var. Genetic basis of the highly efficient yeast kluyveromyces. Intraspecies polymorphisms of kluyveromyces marxianus strains.
This proteome is part of the kluyveromyces lactis strain. Here, we report the draft genome sequence of kluyveromyces marxianus fragilis b0399, the first yeast approved as a probiotic for human consumption not belonging to the genus saccharomyces the. Hello, my name is kluyveromyces marxianus eureka brewing. Kluyveromyces marxianus is a nonconventional thermotolerant yeast with status generally regarded as safe. Pdf draft genome sequence of kluyveromyces marxianus. Its name comes from the ability to assimilate lactose and convert it into lactic acid kluyveromyces lactis formerly saccharomyces lactis is a yeast which has the ability to assimilate lactose and convert it into lactic acid. Kluyveromyces marxianus is a nonconventional yeast whose physiology and metabolism lends itself to diverse biotechnological applications. Kmmig1 as a disrupted mutant of mig1 encoding a regulator for glucose repression in kluyveromyces marxianus exhibits a histidineauxotrophic phenotype. Currently, the poor ethanol tolerance hinders its operable application in the industry, and it is necessary to improve k. Kluyveromyces marxianus strain dmku31042 bcc 29191 nbrc. Kluyveromyces marxianus dmku31042 ensembl genomes 46. Kluyveromyces marxianus is a thermotolerant, crabtreenegative yeast, which preferentially directs metabolism e.
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